بررسی اثرات میدانهای الکترومغناطیس بر حفظ نورونهای گانگلیونهای ریشه خلفی عصب سیاتیک ضایعه دیده در رت

&nbsp;Previous studies have been shown that electromagnetic fields (EMF)&nbsp;result in increasing the rate of nerve regeneration. Therefore it could be&nbsp;assumed following axotomy the signal induction of these fields may protect&nbsp;neural cells from death. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; For this study 24 male wistar rats (2 month age) divided to 4 groups&nbsp;(experimental 1, 2,&nbsp;control&nbsp;and sham).&nbsp;For axotomy, the animals were&nbsp;anesthetized and their right Sciatic Nerve (SN) were exposed and crushed in&nbsp;the mid portion of the thigh in experimental and control groups.&nbsp;In the sham&nbsp;group the SN just exposed (no compression). In the next phase experimental&nbsp;groups were exposed to the&nbsp;50 HZ&nbsp;electeromagnetic field&nbsp;(EMF), 1.1mTl 2-4 &nbsp;&nbsp; hr/day (for 10 days). After 8 weeks, Dorsal Root Ganglions (DRG)s in the&nbsp;lumbar segments (L4-L6) of spinal cord were sampled, processed sectioned&nbsp;serially and stained with toluidine blue (pH=4.5). By using stereological&nbsp;quantitative technique (physical disector), the neurons in the crushed side&nbsp;(DRG)s were counted and compared in the all of groups. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;Statistical analyses of results have been shown a remarkable reduction&nbsp;in neuronal density in (DRG)s of control.&nbsp;On the other hand we observed a&nbsp;cell death DRG significant among experimental groups (p<0/05). In addition&nbsp;we compared DRG volume in all groups but they didn&rsquo;t show any significant &nbsp;&nbsp; difference. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;These findings indicate that the EMF may play a survival role against&nbsp;neuronal death during nerve injuries.